Transient expression of alpha-1B adrenoceptor messenger ribonucleic acids in the rat superior cervical ganglion...

We have examined the developmental profile of the alpha-1 and alpha-2 adrenergic receptor messenger ribonucleic acids expression in the rat superior cervical ganglion. The expression of the six messenger ribonucleic acids was studied using reverse transcription-polymerase chain reaction. At four weeks, the dominant messenger ribonucleic acids transcripts in this sympathetic ganglion were alpha-1C, alpha-2A, alpha-2B and alpha-2C. The expression of alpha-1 genes in the superior cervical ganglion appears to be regulated during postnatal development in that two alpha-1 (alpha-1B, alpha-1C) genes were expressed at birth, three, seven and 14 days postnatal but no amplified product for alpha-1B was detected at 28 days and in the aged animals, while the alpha-1C transcript continued to be expressed. No amplified product for alpha-1D was detected in superior cervical ganglion at any of the ages studied. While all three alpha-2 genes were expressed in the superior cervical ganglion at four weeks the dominant alpha-2 messenger ribonucleic acids transcript expressed in the superior cervical ganglion was alpha-2A. This pattern of alpha-2 adrenoceptor gene expression was maintained from birth, throughout development and into old age. These results suggest that the expression of alpha-1 adrenergic receptors in the superior cervical ganglion is regulated developmentally while the expression of alpha-2 genes remains unchanged.