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Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2
1Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Biochemical and Biophysical Research Communications
|February 24, 1997
Abstract:
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.