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Related Experiment Videos

N,N-dimethylsphingosine phosphorylation in human platelets

Y Yatomi1, Y Ozaki, K Satoh

  • 1Department of Laboratory Medicine, Yamanashi Medical University, Japan.

Biochemical and Biophysical Research Communications
|February 24, 1997
PubMed
Summary

Human platelets metabolize sphingosine derivatives. Sphingosine 1-phosphate formed rapidly, while N,N-dimethylsphingosine 1-phosphate (DMS-1-P) production occurred weakly in stimulated platelets, representing a novel biological finding.

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Inhibition of MAP kinase by sphingosine and its methylated derivative, N,N-dimethylsphingosine.

International journal of oncology·2011

Area of Science:

  • Biochemistry
  • Cell Biology
  • Hematology

Background:

  • Sphingolipids are crucial bioactive molecules involved in cellular signaling and function.
  • Platelets play a key role in hemostasis and thrombosis, with their metabolism being a focus of research.

Purpose of the Study:

  • To investigate the metabolism of sphingosine (Sph) derivatives in human platelets.
  • To characterize the formation and regulation of specific sphingolipid metabolites within platelets.

Main Methods:

  • Utilized radiolabeled substrates ([3H]Sph, [14C]N,N-dimethylsphingosine, [3H]C2-ceramide) to trace metabolic pathways.
  • Examined metabolic activity in both resting and stimulated human platelets.
  • Employed specific activators (thrombin, 12-O-tetradecanoylphorbol 13-acetate) and inhibitors (staurosporine) to study regulatory mechanisms.

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Main Results:

  • Rapid and extensive phosphorylation of [3H]Sph to sphingosine 1-phosphate in both resting and stimulated platelets.
  • [14C]N,N-dimethylsphingosine was stable in resting platelets but weakly converted to N,N-dimethylsphingosine 1-phosphate (DMS-1-P) upon stimulation.
  • DMS-1-P formation was significantly inhibited by staurosporine, indicating a role for protein kinases.
  • [3H]C2-ceramide remained unchanged under all experimental conditions.

Conclusions:

  • Human platelets actively metabolize sphingosine, primarily forming sphingosine 1-phosphate.
  • Platelet activation promotes the formation of N,N-dimethylsphingosine 1-phosphate (DMS-1-P), a novel finding in biological systems.
  • Protein kinase activity is involved in the regulation of DMS-1-P production in platelets.