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Cyclin and cyclin-dependent kinase expression in the remnant glomerulus
S J Shankland1, P Hamel, J W Scholey
1Department of Medicine, University of Toronto, Ontario, Canada.
Journal of the American Society of Nephrology : JASN
|March 1, 1997
Summary
Subtotal renal ablation triggers mesangial cell proliferation by increasing cyclin E and phosphorylated retinoblastoma protein (pRb) in glomeruli, indicating cell cycle progression after kidney injury.
Area of Science:
- Nephrology
- Cell Biology
- Molecular Biology
Background:
- Subtotal renal ablation is linked to early mesangial cell proliferation.
- Mechanisms driving this proliferation remain unclear.
Purpose of the Study:
- Investigate changes in cell cycle regulators (pRb, cyclin E, cdk2) in glomeruli.
- Examine these changes during early compensatory renal hypertrophy post-5/6 ablation.
Main Methods:
- Utilized Western blot for PCNA and pRb protein expression.
- Employed Northern blot for cyclin E and cdk2 mRNA levels.
- Conducted immunohistochemical analysis for PCNA in renal cortex.
Main Results:
- A fivefold increase in PCNA-positive nuclei observed in glomeruli 96 hours post-ablation.
- Appearance of phosphorylated pRb alongside increased PCNA.
- Twofold increase in cyclin E mRNA, while cdk2 mRNA remained unchanged.
Conclusions:
- Renal ablation induces glomerular cell cycle progression.
- Increased cyclin E and phosphorylated pRb are associated with this progression.