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Renal cortical complement C3 gene expression in IgA nephropathy

V Montinaro1, L Gesualdo, E Ranieri

  • 1Institute of Nephrology, University of Bari Polyclinic, Italy.

Journal of the American Society of Nephrology : JASN
|March 1, 1997
PubMed
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Local C3 gene expression in the kidneys is common in immunoglobulin A (IgA) nephropathy. This local C3 production correlates with disease severity and poorer outcomes in IgA nephropathy patients.

Area of Science:

  • Nephrology
  • Immunology
  • Molecular Biology

Background:

  • Glomerular C3 deposits are characteristic of immunoglobulin A (IgA) nephropathy.
  • Inflammation in kidney tissue is associated with complement component gene expression and protein synthesis.

Purpose of the Study:

  • To investigate the role of locally produced complement component 3 (C3) in the pathogenesis of IgA nephropathy.

Main Methods:

  • Analysis of C3 gene expression using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization.
  • Detection of immunoreactive native C3 on cortical tubules via immunoalkaline-phosphatase staining.

Main Results:

  • C3 mRNA was detected in 56% of IgA nephropathy cases, significantly more in moderate-to-severe lesions.

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  • C3 transcript was primarily expressed by tubular cells, interstitial cells, and glomerular parietal epithelial cells.
  • Renal C3 transcription correlated with glomerulosclerosis, intracapillary proliferation, interstitial damage, and proteinuria.

    • Conclusions:

    • The C3 gene is expressed in proximal tubular cells and glomerular crescents in IgA nephropathy.
    • Local C3 gene expression is linked to clinical and histological markers of IgA nephropathy severity and poor prognosis.
    • This suggests a pathogenetic role for local C3 gene transcription and translation in IgA nephropathy.