[Ultraspectrophotometric method of differential determination of DNA and RNA in spermatozoa]

The authors describe a complement of the previously published ultramicrospectro-photometric method for the dosage of nucleic acids. The spermatozoa are scanned by a bundle of U-V. light. It is so possible to obtain the measurement of the U-V. light absorption on a very big number of very small areas. The data are printed very accurately on one histogram. Thereafter spermatozoa are submitted to the action of RNase and DNase and rescanned with U-V. light. The comparison of the histograms allows to estimate the amount of nucleic acids. Further investigations are requested.