[Identification and determination of carbohydrates in biologic liquids by gas phase chromatography. Application to...

The authors describe a method of estimation of blood sugar by gas chromatography using a microsample of blood placed on paper. The estimation of blood sugar by gas chromatography carried out both on the disc of blood and on the same volume of fresh blood, leads to identical results which are superimposable on those obtained by the ortho-toluidine method. The estimations of galactose, fructose and xylose, lead to comparable results to those obtained by classical colorimetric or enzymatic methods. This method is of interest in pediatrics by the small volume of blood required and by the means of transport of the sample towards the laboratory. A certain number of applications are described: Estimation of fasting blood sugar, during a cycle, or after a glucose loading test. Estimation of various sugars, e.g. galactose, xylose, fructose, in presence of glucose during the loading tests.