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Polyamine levels and ODC activity in intestinal-type and diffuse-type gastric carcinoma
F Russo1, M Linsalata, I Giorgio
1Department of Surgery, I.R.C.C.S. S. de Bellis, Scientific Institute for Digestive Diseases, Castellana G., BA, Italy.
Digestive Diseases and Sciences
|March 1, 1997
Summary
Polyamines and ornithine decarboxylase activity are significantly elevated in intestinal-type gastric cancer compared to diffuse-type. This suggests distinct proliferative behaviors and potential therapeutic strategies for these gastric carcinoma subtypes.
Area of Science:
- Gastroenterology
- Oncology
- Biochemistry
Background:
- Gastric carcinomas are classified as intestinal or diffuse types by Lauren's classification.
- Polyamines (putrescine, spermidine, spermine) are crucial for neoplastic growth in gastrointestinal mucosa.
- Ornithine decarboxylase is the rate-limiting enzyme in polyamine metabolism.
Purpose of the Study:
- To investigate differences in polyamine metabolism between intestinal and diffuse type gastric carcinomas.
- To compare polyamine levels and ornithine decarboxylase activity in distinct gastric tumor subtypes.
Main Methods:
- Studied 27 gastric carcinoma patients (10 intestinal, 17 diffuse types).
- Quantified polyamine levels using high-performance liquid chromatography.
- Measured ornithine decarboxylase activity via a radiometric technique.
Main Results:
- Significantly higher polyamine levels were observed in intestinal type gastric carcinoma samples compared to diffuse type.
- Ornithine decarboxylase activity was also significantly elevated in intestinal type samples.
- Intestinal type polyamine levels resembled those found in colorectal adenocarcinoma.
Conclusions:
- Gastric carcinoma subtypes exhibit distinct polyamine metabolism profiles.
- Differences in polyamine metabolism indicate varied proliferative behaviors between intestinal and diffuse types.
- Findings suggest the potential for developing subtype-specific therapeutic strategies for gastric cancer.