[Production of carbon-labelled rubomycin by means of biosynthesis]

The authors obtained 14C-rubomycin biosynthetically with the use of 14C-acetate, 14C-propionate and methionine (methyl-14C) as the label source. It was shown that radiochemically pure 14C-rubomycin may be obtained from a non-purified preparation of radioactive glucose, i. e. a preparation obtained by hydrolysis of chlorella cells grown on a nitrogen-free medium in an atmosphere of 14CO2. It was found that the use of soyabeen meal + glyserol medium for synthesis of the labeled rubomycin from 14C-glucose resulted in a two-fold increase in the specific activity of the antibiotic.