Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Host function specified by Bacillus pumilus plasmid pPL7065

P S Lovett, E J Duvall, M G Bramucci

    Antimicrobial Agents and Chemotherapy
    |September 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Nicotiana chloroplast genome : 7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Pure bacterial isolates that convert p-xylene to terephthalic acid.

    Applied microbiology and biotechnology·2002
    Same author

    Industrial wastewater bioreactors: sources of novel microorganisms for biotechnology.

    Trends in biotechnology·2000
    Same author

    Suppression of TGA mutations in the Bacillus subtilis spoIIR gene by prfB mutations.

    Journal of bacteriology·1998
    Same author

    Leader peptides of inducible chloramphenicol resistance genes from gram-positive and gram-negative bacteria bind to yeast and Archaea large subunit rRNA.

    Nucleic acids research·1997
    Same author

    Translation attenuation regulation of chloramphenicol resistance in bacteria--a review.

    Gene·1996
    Same journal

    Isavuconazole for invasive mold disease in patients with hematological malignancies: a multicenter real-world study from China on efficacy, safety, and competing risks.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    An Achilles' heel for methicillin-resistant <i>Staphylococcus aureus</i>? Re-evaluating β-lactam susceptibility of MRSA.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Essential role of glycogen synthase kinase 3 in regulating growth, drug response, and infectivity in <i>Leishmania infantum</i>.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    <i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

    Antimicrobial agents and chemotherapy·2026
    See all related articles

    Bacillus pumilus strains produce a killing activity and immunity due to plasmid pPL7065. This plasmid is compatible with other plasmids like pPL576, aiding genetic studies.

    Area of Science:

    • Microbiology
    • Bacterial Genetics
    • Molecular Biology

    Background:

    • Bacillus pumilus is a species of bacteria with industrial and biotechnological applications.
    • Plasmids are extrachromosomal DNA molecules that can confer advantageous traits to host bacteria.
    • Understanding plasmid functions is crucial for genetic manipulation and strain improvement.

    Purpose of the Study:

    • To characterize plasmid pPL7065 in Bacillus pumilus.
    • To investigate the role of pPL7065 in determining bacterial killing activity and immunity.
    • To assess the compatibility of pPL7065 with other plasmids.

    Main Methods:

    • Plasmid isolation and characterization from Bacillus pumilus strains.
    • Assays to detect killing activity and immunity conferred by the plasmid.

    Related Experiment Videos

  • Compatibility studies using transformation and selection markers.

    • Main Results:

    • Plasmid pPL7065 (4.7 x 10^6 daltons) was identified in Bacillus pumilus.
    • This plasmid encodes for a killing activity and confers immunity/resistance to it.
    • pPL7065 demonstrated compatibility with plasmid pPL576 (28 x 10^6 daltons).

    Conclusions:

    • Plasmid pPL7065 plays a significant role in Bacillus pumilus by mediating a self-killing/immunity system.
    • The compatibility of pPL7065 with other plasmids, such as pPL576, facilitates its use in genetic engineering and further research.
    • This finding contributes to the understanding of plasmid biology in Bacillus species.