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cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene
S Kumar1, K F Harvey, M Kinoshita
1The Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Rundle Mall, Adelaide, South Australia, 5000, Australia.
Genomics
|March 15, 1997
Summary
The Nedd4 gene, crucial for embryonic brain development, encodes a protein involved in cellular functions. Its expression is highest during neurogenesis and it is localized to the cytoplasm.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- The Nedd4 gene was identified as a highly expressed transcript in the mouse embryonic brain.
- Nedd4 encodes a protein with calcium/phospholipid binding, WW, and HECT domains, suggesting roles in protein-protein interactions and ubiquitination.
Purpose of the Study:
- To characterize the Nedd4 gene and its protein product.
- To investigate the expression pattern and localization of Nedd4 during mouse embryonic development.
- To map the chromosomal location of the mouse Nedd4 gene.
Main Methods:
- Subtraction cloning was used to identify the Nedd4 gene.
- Nedd4 cDNA cloning and sequence analysis were performed.
- Antibody-based detection was used to determine Nedd4 protein localization.
- An interspecific backcross panel was utilized for gene mapping.
Main Results:
- Nedd4 encodes a 103 kDa protein with distinct functional domains.
- Nedd4 expression is highest in the embryonic central nervous system during neurogenesis, decreasing with development.
- Nedd4 protein is localized to the cellular cytoplasm.
- The mouse Nedd4 gene was mapped to chromosome 9.
Conclusions:
- Nedd4 plays a significant role in mouse embryonic development, particularly in the central nervous system.
- The characterized domains suggest Nedd4 functions in protein ubiquitination pathways.
- Nedd4's chromosomal mapping provides insights into comparative genomics between mouse and human chromosomes.