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Glucose metabolism in Acetobacter aceti

J Flückiger, L Ettlinger

    Archives of Microbiology
    |August 26, 1977
    PubMed
    Summary

    Acetobacter aceti cannot grow on glucose due to its inability to metabolize it effectively. Researchers found no pyruvate kinase activity, suggesting a key metabolic pathway is missing in this bacterium.

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    Area of Science:

    • Microbiology
    • Bacterial Metabolism
    • Biochemistry

    Background:

    • Acetobacter aceti NCIB 8554 utilizes ethanol but not glucose as a carbon source.
    • A glucose-sensitive mutant (A5) exhibits growth inhibition in the presence of glucose.

    Purpose of the Study:

    • To investigate the glucose metabolism pathways in wild-type and mutant Acetobacter aceti.
    • To identify the metabolic bottlenecks preventing glucose utilization.

    Main Methods:

    • Radiorespirometric experiments with a custom-built continuous substrate feeding apparatus.
    • Enzymatic assays to detect key metabolic enzymes.
    • Glucose uptake experiments.

    Main Results:

    • Acetobacter aceti excretes approximately 30% of internalized glucose as gluconate and metabolizes 6% to CO2.
    • The remaining glucose accumulates intracellularly without significant differences between wild-type and mutant strains.
    • Pyruvate kinase activity (EC 2.7.1.40) was undetectable under various conditions.

    Conclusions:

    • The absence of detectable pyruvate kinase activity is a likely explanation for Acetobacter aceti's inability to grow on glucose.
    • Glucose metabolism in Acetobacter aceti involves significant gluconate excretion and intracellular accumulation, rather than complete catabolism.

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