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Related Experiment Videos

2006 expressed-sequence tags derived from human chromosome 7-enriched cDNA libraries

J W Touchman1, G G Bouffard, L A Weintraub

  • 1Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Genome Research
|March 1, 1997
PubMed
Summary

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Researchers generated over 2000 expressed sequence tags (ESTs) for human chromosome 7. This provides valuable gene sequences and 180 new sequence-tagged sites (STSs) for chromosome 7 mapping efforts.

Area of Science:

  • Genomics
  • Human Genetics
  • Molecular Biology

Background:

  • Gene mapping and sequencing are crucial for understanding human chromosomes.
  • Chromosome 7 research requires specific gene sequence data.

Purpose of the Study:

  • To generate and analyze expressed sequence tags (ESTs) for human chromosome 7.
  • To develop sequence-tagged sites (STSs) for chromosome 7 gene mapping.
  • To demonstrate the effectiveness of direct selection for enriching chromosome-specific cDNAs.

Main Methods:

  • Generated 2006 ESTs from direct selection cDNA libraries enriched for chromosome 7.
  • Performed similarity searches against public databases.
  • Developed 190 human DNA-specific STSs from ESTs.
  • Mapped STSs to chromosome 7 and assigned them to YAC contigs.

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Main Results:

  • Two-thirds of ESTs were novel, not found in public databases.
  • 68% of ESTs were unique within the generated collection.
  • 180 of 190 developed STSs (96%) mapped to chromosome 7.
  • 140 EST-specific STSs were assigned to YAC contigs across chromosome 7.

Conclusions:

  • The study yielded over 2000 chromosome 7-enriched ESTs and 180 new STSs.
  • Direct selection is a robust method for enriching chromosome-specific cDNAs.
  • The data will aid in constructing a comprehensive chromosome 7 transcript map.