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Conjugated linoleic acid modulates hepatic lipid composition in mice
1Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907, USA.
Lipids
|February 1, 1997
Summary
Dietary conjugated linoleic acid (CLA) alters liver lipid metabolism in mice. CLA incorporation into liver lipids reduced linoleate and arachidonate, suggesting a mechanism for its chemoprotective effects.
Area of Science:
- Biochemistry
- Nutritional Science
- Cancer Research
Background:
- Conjugated linoleic acid (CLA) demonstrates chemoprotective properties against various cancers in animal models.
- The mechanism of CLA's extrahepatic chemoprotective effects is hypothesized to involve modulation of liver lipid metabolism.
Purpose of the Study:
- To investigate the role of dietary CLA in modulating liver fatty acid composition and metabolism.
- To determine how CLA affects lipid profiles in hepatic tissues.
Main Methods:
- Female SENCAR mice were fed diets with varying CLA concentrations (0.0% to 1.5%) for six weeks.
- Liver lipid content and fatty acid composition were analyzed using gas chromatography.
- In vitro assays assessed CLA desaturation compared to linoleate.
Main Results:
- Mice fed CLA exhibited lower body weights and increased liver total lipid content.
- Dietary CLA incorporation into liver phospholipids and neutral lipids occurred at the expense of linoleate.
- Oleate levels increased, while arachidonate levels decreased in neutral lipids of CLA-fed mice.
- CLA desaturation in vitro yielded an unidentified 18:3 product.
Conclusions:
- Dietary CLA significantly alters hepatic fatty acid composition, reducing linoleate and arachidonate levels.
- These metabolic changes in the liver may underlie CLA's observed chemoprotective effects in extrahepatic tissues.
- Further research is needed to identify CLA metabolites and their role in eicosanoid synthesis.