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Capillary electrophoresis of supercoiled and linear DNA in dilute hydroxyethyl cellulose solution
R W Hammond1, H Oana, J J Schwinefus
1Department of Chemistry, School of Medicine, University of Michigan, Ann Arbor 48109, USA.
Analytical Chemistry
|March 15, 1997
Abstract:
Capillary electrophoresis in dilute hydroxyethyl cellulose is shown to separate supercoiled DNA in the size range 2000-16,000 base pairs. The plasmids migrate more slowly than linear ds-DNA of the same sizes. Plasmid bandwidths are larger than observed for ds-DNA, allowing identification of the type of DNA by bandwidth. The differing dependence of mobility on chain length can be explained by assuming that a plasmid migrates as an elastic rod, while ds-DNA migrates as a wormlike chain.