Related Experiment Videos
Adenosine deaminase activity in nephrotic syndrome
1Department of Pediatrics, Banaras Hindu University, India.
Journal of Tropical Pediatrics
|February 1, 1997
Summary
Children with nephrotic syndrome show impaired cell-mediated immunity (CMI), with reduced T-lymphocytes and elevated adenosine deaminase (ADA) activity. These immune changes persist even during remission, indicating a chronic immune dysfunction.
Area of Science:
- Immunology
- Pediatrics
- Nephrology
Background:
- Idiopathic nephrotic syndrome (INS) is a significant renal disorder in children.
- Cell-mediated immunity (CMI) plays a crucial role in immune defense and its dysfunction is implicated in various diseases.
Purpose of the Study:
- To evaluate the cell-mediated immunity status in children with idiopathic nephrotic syndrome.
- To assess changes in CMI parameters during active disease and remission phases.
- To investigate the role of adenosine deaminase (ADA) activity as a potential biomarker.
Main Methods:
- Assessed CMI by measuring response to purified protein derivative (PPD).
- Quantified absolute lymphocyte count (ALC) and T-lymphocytes percentage.
- Measured serum adenosine deaminase (ADA) activity in patients and healthy controls.
Main Results:
- Patients with active nephrotic syndrome exhibited decreased PPD response, ALC, and T-lymphocytes percentage, alongside elevated ADA activity compared to controls.
- A significant negative correlation was found between T-lymphocytes percentage and ADA activity.
- While ALC and T-lymphocytes improved in remission, they remained lower than controls; ADA activity showed no significant change from the active phase.
Conclusions:
- Children with INS demonstrate impaired CMI in both active and remission stages.
- Elevated ADA activity is a prominent finding, suggesting its potential as a sensitive indicator of immune dysfunction in INS.
- CMI deficits persist even after clinical remission, highlighting the long-term immunological impact of the disease.