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Related Experiment Videos

On-pathway versus off-pathway folding intermediates

R L Baldwin1

  • 1Biochemistry Department, Beckman Center, Stanford University Medical Center, CA 94305-5307, USA.

Folding & Design
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

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Molten globule intermediates in protein folding are debated as on-pathway or off-pathway. Recent experiments explore their role, stability, and implications for protein folding mechanisms.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Dynamics

Background:

  • Molten globule intermediates (MGIs) are transient states observed during protein folding.
  • The precise role of MGIs (on-pathway vs. off-pathway) in protein folding remains a subject of debate.
  • Understanding MGI behavior is crucial for elucidating protein folding mechanisms.

Purpose of the Study:

  • To review and discuss recent experimental evidence regarding the nature of molten globule intermediates in protein folding.
  • To address the controversy surrounding whether these intermediates are on the direct folding pathway or represent off-pathway species.
  • To explore the implications of MGI stability and observation for different protein folding models.

Main Methods:

  • Analysis of kinetic data, including folding rates as a function of denaturant concentration.

Related Experiment Videos

  • Interpretation of experimental observations related to the stability and detectability of folding intermediates.
  • Review of studies investigating specific cases like hen lysozyme and the search for unfolding intermediates.

    • Main Results:

    • The presence or absence of a 'rollover' in folding rate versus denaturant plots is analyzed for its implication on intermediate pathway status.
    • The lack of observable intermediates for some proteins is discussed in terms of alternative folding mechanisms or reduced intermediate stability.
    • Recent findings concerning unfolding intermediates are considered in the context of the overall protein folding landscape.

    Conclusions:

    • Experimental evidence is presented to aid in resolving the on-pathway versus off-pathway debate for molten globule intermediates.
    • The stability and detectability of intermediates significantly influence the interpretation of protein folding pathways.
    • Further research into unfolding intermediates may provide complementary insights into the protein folding process.