Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid hormone-dependent gene expression program for Xenopus neural development

R J Denver1, S Pavgi, Y B Shi

  • 1Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109, USA. rdenver@umich.edu

The Journal of Biological Chemistry
|March 28, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of a wide-energy-range nano-focus and high-resolution soft x-ray beamline.

The Review of scientific instruments·2025
Same author

Exceptional spectrum and dynamic magnetization.

Journal of physics. Condensed matter : an Institute of Physics journal·2022
Same author

Approach for removing ghost-images in remote field eddy current testing of ferromagnetic pipes.

The Review of scientific instruments·2016
Same author

[Temporal bone chondrosarcoma: report of one case].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2016
Same author

Risk factors for pulmonary hypertension in patients receiving maintenance peritoneal dialysis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2016
Same author

Role of fibroblast growth factor-23 in the pathogenesis of atherosclerosis in peritoneal dialysis patients.

Genetics and molecular research : GMR·2015
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

Researchers identified 34 thyroid hormone (TH)-regulated genes in the developing Xenopus brain. This discovery provides new tools to understand how TH influences neural development and gene regulation in vertebrates.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Endocrinology

Background:

  • Thyroid hormone (TH) is crucial for vertebrate neural development.
  • The precise molecular mechanisms of TH action in the brain remain largely unknown.

Purpose of the Study:

  • To identify novel genes regulated by thyroid hormone in the developing vertebrate brain.
  • To elucidate the molecular pathways involved in TH-mediated neural development.

Main Methods:

  • Polymerase chain reaction-based subtractive hybridization was used to isolate TH-regulated cDNAs from Xenopus tadpole diencephalon.
  • Northern blot analysis confirmed TH regulation and metamorphic expression of identified mRNAs.
  • Gene sequencing and homology searches identified the functions of isolated cDNA fragments.

Related Experiment Videos

Main Results:

  • 34 cDNAs for TH-regulated genes were isolated from the Xenopus diencephalon.
  • Most identified genes were upregulated by TH within 4-8 hours, with 13 genes showing brain-specific regulation.
  • Identified genes include transcription factors, a deiodinase, metabolic enzymes, and neuropeptides, offering insights into TH-target gene networks.

Conclusions:

  • This study represents the first large-scale isolation of TH-target genes in the developing vertebrate brain.
  • The identified genes provide a foundation for understanding gene regulatory networks governing TH's role in neural development.
  • The cloned cDNAs serve as valuable tools for investigating molecular mechanisms of neural cell differentiation.