Differential regulation of actin depolymerizing factor and cofilin in response to alterations in the actin monomer...

Myoblasts, transfected with a human gene encoding a beta-actin point mutation, down-regulate expression of actin depolymerizing factor (ADF) and its mRNA. Regulation is posttranscriptional. Expression of cofilin, a structurally similar protein, and profilin, CapG, and tropomodulin is not altered with increasing mutant beta-actin expression. Myoblasts expressing either human gamma-actin or the mutant beta-actin down-regulate the endogenous mouse actin genes to keep a constant level of actin mRNA, whereas the gamma-actin transfectants do not down-regulate ADF. Thus, ADF expression is regulated differently from actin expression. The mutant beta-actin binds to ADF with about the same affinity as normal actin; however, it does not assemble into normal actin filaments. The decrease in ADF expression correlates with an increase in the unassembled actin pool. When the actin monomer pool in untransfected myoblasts is increased 70% by treatment with latrunculin A, synthesis of ADF and actin are down-regulated compared with cofilin and 19 other proteins selected at random. Increasing the actin monomer pool also results in nearly complete phosphorylation of both ADF and cofilin. Thus, ADF and cofilin are coordinately regulated by posttranslational modification, but their expression is differentially regulated. Furthermore, expression of ADF is responsive to the utilization of actin by the cell.