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Transcripts for functionally distinct isoforms of chicken GATA-5 are differentially expressed from alternative first
C MacNeill1, B Ayres, A C Laverriere
1Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
The Journal of Biological Chemistry
|March 28, 1997
Summary
Chicken GATA-5 gene expression utilizes alternative first exons, producing two distinct GATA-5 protein isoforms. One isoform has reduced DNA-binding zinc fingers and distinct transactivation function.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- The GATA-5 transcription factor plays a role in vertebrate development.
- Previous studies characterized a major isoform of chicken GATA-5.
Purpose of the Study:
- To investigate the expression and function of chicken GATA-5.
- To identify novel isoforms of chicken GATA-5.
Main Methods:
- Analysis of cDNA and genomic clones.
- mRNA transcript analysis.
- Transfection studies in cultured cells.
Main Results:
- Chicken GATA-5 is expressed from alternative first exons, yielding two distinct mRNA transcripts.
- These transcripts encode two GATA-5 isoforms: a major isoform with two zinc fingers and a novel minor isoform with one zinc finger.
- The minor GATA-5 isoform localizes to the nucleus, binds GATA DNA sites, but exhibits reduced transactivation potential compared to the major isoform.
Conclusions:
- Differential expression and processing of the chicken GATA-5 gene generate functionally distinct isoforms.
- The novel, truncated GATA-5 isoform represents a distinct functional variant with altered transcriptional activity.