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Large-scale plant cell culture

Roberts1, Shuler

  • 1School of Chemical Engineering 120 Olin Hall Cornell University Ithaca, NY 14853-5201, USA

Current Opinion in Biotechnology
|April 1, 1997
PubMed
Summary

Plant cell culture technology has advanced significantly, boosting secondary metabolite production through elicitation with methyl jasmonate and synergistic strategies. This progress, exemplified by paclitaxel (Taxol) yield increases, enables more efficient commercial-scale biomanufacturing.

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Area of Science:

  • Biotechnology and Plant Science
  • Metabolic Engineering
  • Natural Product Synthesis

Background:

  • Commercial-scale plant cell culture applications have seen substantial progress.
  • Secondary metabolite production is crucial for various industries.
  • Understanding biosynthetic pathways is key to optimizing yields.

Purpose of the Study:

  • To highlight recent advancements in plant cell culture for commercial applications.
  • To detail effective strategies for enhancing secondary metabolite production.
  • To showcase the transition towards semirational enhancement approaches.

Main Methods:

  • Elicitation using methyl jasmonate.
  • Synergistic application of enhancement strategies: immobilization and in situ extraction.
  • Leveraging advances in understanding secondary metabolite biosynthetic pathway regulation.

Main Results:

  • Significant increases in product yields for diverse secondary metabolites.
  • Methyl jasmonate elicitation proves effective, especially with synergistic strategies.
  • Over 100-fold yield improvement for paclitaxel (Taxol) in the last two years.

Conclusions:

  • Plant cell cultures are nearing commercial viability due to enhanced production methods.
  • Semirational design of enhancement strategies is becoming feasible.
  • Optimized elicitation and extraction techniques drive significant yield improvements.

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