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A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli
W Jäger1, J Kalinowski, A Pühler
1Lehrstuhl für Genetik, Universität Bielefeld, Germany.
Journal of Bacteriology
|April 1, 1997
Abstract:
A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.