Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging the hierarchical Ca2+ signalling system in HeLa cells

M Bootman1, E Niggli, M Berridge

  • 1Department of Physiology, University of Bern, Switzerland.

The Journal of Physiology
|March 1, 1997
PubMed
Summary

This study reveals a hierarchy of calcium (Ca2+) signals in non-excitable cells, from elementary Ca2+ blips and puffs to complex Ca2+ waves. These findings provide a prototype for understanding Ca2+ signal transduction in these cell types.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is there a role of C-reactive protein in red blood cell aggregation?

International journal of laboratory hematology·2014
Same author

Study on the removal efficiency of UF membranes using bacteriophages in bench-scale and semi-technical scale.

Water science and technology : a journal of the International Association on Water Pollution Research·2012
Same author

Consideration of emerging pollutants in groundwater-based reuse concepts.

Water science and technology : a journal of the International Association on Water Pollution Research·2012
Same author

Pathways of abnormal stress-induced Ca2+ influx into dystrophic mdx cardiomyocytes.

Cell calcium·2009
Same author

Ca2+ release from the sarcoplasmic reticulum activated by the low affinity Ca2+ chelator TPEN in ventricular myocytes.

Cell calcium·2006
Same author

Confocal imaging of CICR events from isolated and immobilized SR vesicles.

Cell calcium·2005

Area of Science:

  • Cellular Biology
  • Biophysics
  • Physiology

Background:

  • Calcium ions (Ca2+) are crucial intracellular second messengers.
  • Understanding Ca2+ signaling in non-excitable cells is vital for various physiological processes.
  • The endoplasmic reticulum (ER) is a primary storage site for intracellular Ca2+.

Purpose of the Study:

  • To investigate hormone-induced subcellular Ca2+ release signals from the ER in HeLa cells.
  • To characterize the elementary Ca2+ signals (blips and puffs) and their relationship to Ca2+ waves.
  • To elucidate the mechanisms of Ca2+ wave propagation.

Main Methods:

  • Confocal microscopy was employed to visualize and quantify Ca2+ signals.
  • Histamine was used as a stimulus to evoke Ca2+ release.

Related Experiment Videos

  • Analysis of Ca2+ signal kinetics, amplitude, and spatial spreading.

    • Main Results:

    • Two types of elementary Ca2+ signals were identified: Ca2+ blips (from single channels) and Ca2+ puffs (from multiple channels).
    • Ca2+ waves originated from stable initiation sites and their characteristics depended on the number of recruited puffs.
    • Wave propagation involves diffusion and regeneration, with threshold stimulation leading to abortive waves.

    Conclusions:

    • A hierarchical model of Ca2+ signaling (blips -> puffs -> waves) is proposed for non-excitable cells.
    • This signaling cascade is analogous to Ca2+ events observed in cardiac muscle cells.
    • The findings offer insights into Ca2+ signal transduction mechanisms in a broad range of cell types.