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Updated: Aug 19, 2026

A General Method for Evaluating Deep Brain Stimulation Effects on Intravenous Methamphetamine Self-Administration
Published on: January 22, 2016
c-fos antisense blocks methamphetamine-induced ambulatory activity reversibly
T Umekage1, M Namima, K Fukushima
1Department of Pharmacology, National Defense Medical College, Tokorozawa, Japan.
Abstract:
To investigate the involvement of immediate early genes (IEGs) in the ambulatory activity induced by a low dose of methamphetamine (MAP) in mice, we injected an antisense phosphorothioate oligodeoxynucleotide (ODN) to c-fos mRNA stereotactically into the striatum of ddY male mice. MAP (2 mg kg-1)-induced ambulatory activity was significantly suppressed when the mouse was pretreated by the ODN (2 mM, 1 microliter), and this was accompanied by a decrease in c-Fos-like protein immunoreactivity. Repetitive intermittent administration of MAP at intervals of 3 days confirmed that the suppressive effect was transient and reproducible. These results suggest that c-Fos is at least partly the key molecule regulating the psychomotor stimulation by MAP in mouse brain.
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