Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[DNA degradation in formalin fixed tissues]

P Wiegand1, J Domhöver, B Brinkmann

  • 1Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Münster.

Der Pathologe
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Old meets new: Comparative examination of conventional and innovative RNA-based methods for body fluid identification of laundered seminal fluid stains after modular extraction of DNA and RNA.

Forensic science international. Genetics·2018
Same author

Comprehensive examination of conventional and innovative body fluid identification approaches and DNA profiling of laundered blood- and saliva-stained pieces of cloths.

International journal of legal medicine·2017
Same author

[Patio repair for urethrocutaneous fistulae : Results of a multicentre retrospective study].

Der Urologe. Ausg. A·2017
Same author

The Necessity of Developing New Criteria for Carcinogen Classification of Fibers by the Mak Commission.

Inhalation toxicology·2015
Same author

Fatal mycotic aneurysm caused by Pseudallescheria boydii after near drowning.

International journal of legal medicine·2009
Same author

Does breastfeeding reduce the risk of sudden infant death syndrome?

Pediatrics·2009

DNA degradation in fixed tissues increases with formalin concentration and time. However, even heavily degraded samples from muscle, brain, liver, and bone tissues were successfully individualized using PCR analysis.

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Histology

Context:

  • Tissue fixation is crucial for preserving biological samples.
  • Formalin fixation duration and concentration significantly impact DNA integrity.
  • Understanding DNA degradation is vital for forensic and research applications.

Purpose:

  • To investigate DNA degradation in various tissues fixed for extended periods (up to 70 days) with different unbuffered formalin concentrations (2%, 4%, 8%).
  • To assess the impact of fixation parameters on DNA extractability and integrity.
  • To determine the efficacy of Polymerase Chain Reaction (PCR) analysis for individualizing DNA from fixed tissues.

Summary:

  • DNA yield decreased and degradation increased with higher formalin concentrations and longer fixation times.

Related Experiment Videos

  • Muscle and bone tissues yielded more DNA than liver and brain tissues.
  • Despite degradation, all fixed tissue samples were successfully typed using the PCR-VNTR system HUMTH01, regardless of fixation conditions.

    • Impact:

    • This study highlights the resilience of DNA for individualization even after prolonged formalin fixation.
    • Provides critical data for optimizing tissue fixation protocols in forensic casework and research.
    • Demonstrates the robustness of PCR-VNTR analysis for DNA profiling from challenging samples.