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Outer membrane proteins and elastase of Pseudomonas aeruginosa after the postantibiotic effect induced by amikacin
1Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Abstract:
Postantibiotic effects were induced in three Pseudomonas aeruginosa strains after short-term treatment (30 min) with amikacin at suprainhibitory concentrations in the range of 0.6-1.2 h (2 x MIC) and of 3.0-4.4 h (4 x MIC). Significant suppression of P. aeruginosa elastase activity was found after treatment with amikacin at 4 x MIC concentration (to 10-69.2% of the control values); 2 x MIC was less effective. The profile of outer membrane proteins of P. aeruginosa was not changed after treatment with amikacin at both concentrations.
Insights
Amikacin treatment induced postantibiotic effects in Pseudomonas aeruginosa. Higher amikacin concentrations (4x MIC) significantly suppressed elastase activity, but outer membrane protein profiles remained unchanged.
Area of Science:
- Microbiology
- Pharmacology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen.
- Understanding antibiotic effects is crucial for treatment.
Purpose of the Study:
- To investigate postantibiotic effects of amikacin on Pseudomonas aeruginosa.
- To assess the impact of amikacin on elastase activity and outer membrane proteins.
Main Methods:
- Exposure of P. aeruginosa strains to amikacin at 2x and 4x MIC for 30 minutes.
- Measurement of postantibiotic effect duration.
- Assay of elastase activity.
- Analysis of outer membrane protein profiles.
Main Results:
- Postantibiotic effects were observed, lasting 0.6-1.2 h (2x MIC) and 3.0-4.4 h (4x MIC).
- Amikacin at 4x MIC significantly suppressed P. aeruginosa elastase activity (10-69.2% of control).
- Outer membrane protein profiles were unaffected by amikacin treatment at both concentrations.
Conclusions:
- Amikacin exhibits postantibiotic effects on P. aeruginosa.
- Higher amikacin concentrations are more effective in suppressing elastase activity.
- Amikacin does not alter the outer membrane protein profile of P. aeruginosa.