Related Experiment Videos
Application of the gel test in IgG subclassing--a comparison of two agglutination assays
1University Hospital, Linköping, Sweden.
Background And Objectives:
IgG subclasses of anti-D seem to play some role in hemolytic disease of the newborn, but there has been disagreement as to its exact nature. The aim of our study was to evaluate a new technique for IgG subclassing of anti-D and to compare it to an established test.
Materials And Methods:
In 31 cases of RhD immunization, we have compared two simple agglutinating assays for subclassing anti-D: an established V-well microtiter tray assay and a new gel test assay. Polyclonal rabbit anti-IgG agglutinating antibodies were tested against sensitized D-positive red blood cells by both assays.
Results:
The anti-D concentration in tested sera varied between 0.1 and 81 micrograms/ml (0.5-405 IU/ml). Both assays were simple to perform and the results correlated well.
Conclusions:
The gel test had two advantages: quick performance and easy interpretation.