Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved method for measuring blood mercaptans

W M Doizaki, L Zieve

    The Journal of Laboratory and Clinical Medicine
    |November 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of increases in ornithine decarboxylase and putrescine has no effect on rat liver regeneration.

    The American journal of physiology·1992
    Same author

    Aging alters ornithine decarboxylase and decreases polyamines in regenerating rat liver but putrescine replacement has no effect.

    The Journal of laboratory and clinical medicine·1992
    Same author

    Aging is associated with reduced liver regeneration and diminished thymidine kinase mRNA content and enzyme activity in the rat.

    The Journal of laboratory and clinical medicine·1991
    Same author

    Ultrastructural study of hepatic regeneration following one-lobe, two-lobe, and subtotal hepatectomy in the rat.

    Experimental pathology·1990
    Same author

    Effects of partial and sham hepatectomy on ornithine decarboxylase and thymidine kinase activities and mRNA contents.

    Biochemistry international·1990
    Same author

    An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver.

    Cell differentiation and development : the official journal of the International Society of Developmental Biologists·1989
    Same journal

    Studies on streptomycin; assay in body fluids.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    Gastrocolic fistula; an experimental study.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    An isolated heart perfusion system adapted to the determination of nongaseous metabolities; with sample data upon the isolated monkey heart.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A new type of glass cage for metabolism studies.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A simple electrocardiograph contact electrode for use with the smaller laboratory animal.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A primary standard for the colorimetric determination of hemoglobin.

    The Journal of laboratory and clinical medicine·2010
    See all related articles

    A new method accurately measures metallothionein (MT) in blood, detecting previously undetectable forms. Elevated MT levels were observed in rats and humans with severe liver conditions, indicating its potential as a biomarker.

    Area of Science:

    • Biochemistry
    • Toxicology
    • Clinical Chemistry

    Background:

    • Metallothionein (MT) is a crucial protein involved in heavy metal detoxification and oxidative stress response.
    • Previous methods for MT blood measurement were limited, unable to detect certain forms or quantify levels in patients.

    Purpose of the Study:

    • To develop and validate an improved, highly sensitive method for measuring metallothionein (MT) in whole blood.
    • To assess blood MT levels in animal models and human patients with severe liver disease.

    Main Methods:

    • Utilized zinc as a reducing agent in conjunction with a more sensitive gas chromatograph for MT quantification.
    • Measured both previously undetectable covalently bound MT and total MT in whole blood samples.

    Related Experiment Videos

    Main Results:

    • Established average normal whole blood MT levels in rats (200 +/- 42 pmol/ml) and humans (229 +/- 29 pmol/ml).
    • Observed significantly elevated blood MT levels (4-18 times normal in rats, 4-5 times normal in humans) in subjects with experimental hepatic coma and cirrhotic encephalopathy.

    Conclusions:

    • The novel method allows for the first time the measurement of covalently bound MT in human blood.
    • Significantly increased blood MT concentrations correlate with severe hepatic conditions, suggesting MT's role and potential as a biomarker in liver disease.