Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laminins in lung development

L Schuger1

  • 1Department of Pathology and Laboratory Medicine, Wayne State University, School of Medicine, Detroit, MI 48201, USA.

Experimental Lung Research
|March 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Allogeneic cell-mediated immunotherapy of leukemia with immune donor lymphocytes to upregulate antitumor effects and downregulate antihost responses.

Bone marrow transplantation·2003
Same author

Mesenchymal-epithelial interactions in lung development and repair: are modeling and remodeling the same process?

American journal of physiology. Lung cellular and molecular physiology·2002
Same author

Maspin expression inversely correlates with breast tumor progression in MMTV/TGF-alpha transgenic mouse model.

Oncogene·2001
Same author

Heterogeneous nuclear ribonucleoprotein-H plays a suppressive role in visceral myogenesis.

Mechanisms of development·2001
Same author

Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia.

The Journal of clinical investigation·2000
Same author

Anti-DNA antibodies cross-reacting with laminin inhibit trophoblast attachment and migration: implications for recurrent pregnancy loss in SLE patients.

American journal of reproductive immunology (New York, N.Y. : 1989)·2000

Laminin-1's distinct molecular regions promote lung development by regulating epithelial cell proliferation, basement membrane formation, and lumen creation. Specific sites are crucial for normal lung structure and branching morphogenesis.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Laminins are crucial basement membrane glycoproteins involved in cell adhesion, proliferation, and differentiation.
  • Laminin-1, identified in mouse tumors, is vital for lung development, specifically branching morphogenesis.
  • Laminins are multidomain proteins with distinct functional sites.

Purpose of the Study:

  • To investigate the specific functions of different laminin-1 domains in lung organogenesis.
  • To elucidate how distinct laminin-1 sites contribute to epithelial cell behavior and basement membrane formation.

Main Methods:

  • Analysis of laminin-1 structure-function relationships in lung development.
  • Investigating the roles of specific laminin-1 regions (cross region, globular regions) in cellular processes.

Related Experiment Videos

Main Results:

  • The cross region of laminin-1 selectively enhances epithelial cell proliferation.
  • Outer globular regions of alpha 1 and beta 1 chains mediate laminin polymerization, basement membrane formation, and cell polarization.
  • The inner globular region of laminin beta 1, along with heparan sulfate proteoglycan binding, stimulates lumen formation.

Conclusions:

  • Different domains of laminin-1 perform distinct, essential functions during lung organogenesis.
  • Proper lung tissue structure and branching morphogenesis depend on the integrated action of these laminin-1 sites.
  • Alterations in specific laminin-1 active sites can lead to lung developmental abnormalities.