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Updated: Aug 12, 2026

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
Published on: December 4, 2012
There is no relationship between concentration, motility and vacuolated cells
S Aktaş1, S Ozkan, P L Cisneros
1Baylor College of Medicine, Reproductive Center of Obstetrics and Gynaecology, Houston, Texas, USA.
Semen analysis revealed no correlation between sperm concentration, motility, and vacuolated sperm cells in fertile donors, infertile patients, or randomized men. These factors do not appear to influence each other in assessing sperm quality.
Area of Science:
- Reproductive biology
- Andrology
- Spermatozoa morphology
Background:
- Vacuolated spermatozoa are observed in semen samples.
- The clinical significance of vacuolated sperm cells in relation to sperm concentration and motility is not fully understood.
Purpose of the Study:
- To investigate potential differences in sperm concentration and motility among vacuolated spermatozoa.
- To compare these parameters in fertile donors, infertile patients, and a group of randomized men.
Main Methods:
- Semen samples were categorized into three groups based on concentration (>20 x 10(6)/ml or <20 x 10(6)/ml) and motility (>50% or <50%).
- Morphological assessment was performed using the strict criteria of Kruger et al.
- Statistical comparisons were made between the groups.
Main Results:
- No significant differences were found between the fertile donor group (Group I) and the infertile patient group (Group II).
- Significant differences were observed between the randomized men group (Group III) and both Group II and Group I.
- The study found no relationship between sperm concentration, motility, and the presence of vacuolated cells.
Conclusions:
- Spermatozoa vacuolation does not appear to be directly related to sperm concentration or motility.
- The observed differences in Group III suggest potential confounding factors or specific characteristics of this group.
- Further research may be needed to elucidate the precise role of vacuolated sperm cells in male fertility.
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