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Related Experiment Videos

Cardiac mutant salamanders: evidence for heart induction

R R Kulikowski, F J Manasek

    The Journal of Experimental Zoology
    |September 1, 1977
    PubMed
    Summary

    Homozygosity for gene c in the Mexican axolotl (Ambystoma mexicanum) prevents heartbeat in the embryo. However, isolated heart tissue rapidly initiates beating, suggesting the issue lies within the embryonic environment, not the heart itself.

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    Area of Science:

    • Developmental biology
    • Cardiovascular research
    • Axolotl genetics

    Background:

    • Homozygosity for gene c in Ambystoma mexicanum is linked to cardiac defects.
    • Previous studies suggest potential embryonic induction failures in cardiac development.

    Purpose of the Study:

    • To investigate the cause of absent heartbeat in Ambystoma mexicanum embryos with gene c homozygosity.
    • To determine if the defect is intrinsic to the cardiac tissue or due to the embryonic environment.

    Main Methods:

    • Organ culture of Ambystoma mexicanum embryos.
    • In situ observation of cardiac function.
    • Isolation and culture of cardiac tissue.

    Main Results:

    • Embryos with gene c homozygosity exhibit no detectable heartbeat in situ.
    • Isolated cardiac tissue from these embryos initiates spontaneous heartbeat rapidly in organ culture.
    • This indicates the cardiac environment, not embryonic induction, is responsible for the lack of function.

    Conclusions:

    • The absence of heartbeat in Ambystoma mexicanum embryos with gene c homozygosity is not due to a failure of embryonic induction.
    • The embryonic cardiac environment plays a critical role in regulating heart function during development.

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