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Presynaptic alpha 2A/D-autoreceptors in the brain cortex of Cercopithecus aethiops
A U Trendelenburg1, I Sutej, K Starke
1Pharmakologisches Institut, Freiburg, Germany.
Naunyn-Schmiedeberg'S Archives of Pharmacology
|March 1, 1997
Summary
Presynaptic alpha 2-autoreceptors in the African green monkey brain cortex were identified. These autoreceptors primarily belong to the alpha 2A/D subtype, showing pharmacological similarities to the alpha 2D subtype.
Area of Science:
- Neuropharmacology
- Adrenergic Receptor Research
Background:
- Presynaptic alpha 2-autoreceptors regulate neurotransmitter release.
- Understanding their subtype is crucial for neurochemical research.
Purpose of the Study:
- To identify the subtype of presynaptic alpha 2-autoreceptors in the brain cortex of African green monkeys (Cercopithecus aethiops).
- To characterize the pharmacological properties of these autoreceptors.
Main Methods:
- Brain cortex slices were incubated with 3H-noradrenaline.
- Electrical stimulation and superfusion with desipramine were used.
- Concentration-response curves of nine alpha-adrenoceptor antagonists were determined to assess autoreceptor affinity.
Main Results:
- All tested antagonists enhanced stimulation-evoked noradrenaline overflow, confirming the presence of presynaptic alpha 2-autoreceptors.
- Affinity estimates indicated that these autoreceptors belong to the alpha 2A/D genetic subtype, not alpha 2B or alpha 2C.
- Pharmacological properties aligned most closely with the alpha 2D subtype.
Conclusions:
- Presynaptic alpha 2-autoreceptors in the African green monkey brain cortex are predominantly of the alpha 2A/D subtype.
- These findings support the classification of these autoreceptors within the alpha 2A/D group of alpha 2-adrenoceptors.