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Methanol-precipitated, detergent-treated Newcastle disease virus as an agar-gel precipitation test antigen
P Roy1, A Koteeswaran, A T Venugopalan
1Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madras, India.
Tropical Animal Health and Production
|February 1, 1997
Abstract:
Methanol-precipitated, detergent-treated (Triton X-100) Newcastle disease virus (NDV) was found suitable as an agar gel precipitation test (AGPT) antigen. One hundred and twenty sera were tested by the haemagglutination-inhibition (HI) test and AGPT against NDV. There was a very significant increase in the proportion of AGPT positive samples with increase in HI titre. Hence AGPT can be recommended as a field based test for seromonitoring following vaccinations against ND where laboratory facilities are inadequate.