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Eicosanoids in bovine retinal microcirculation

P Kulkarni1, S Payne

  • 1University of Louisville School of Medicine, Department of Ophthalmology and Visual Sciences, Kentucky, USA.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
|April 1, 1997
PubMed
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Prostaglandin F2 alpha and U46619 cause vasoconstriction in bovine retinal microcirculation. These effects are mediated by thromboxane A2 (TXA2) receptors, as shown by the antagonist SQ 29,548.

Area of Science:

  • Ophthalmology
  • Vascular Biology
  • Endocrinology

Background:

  • The bovine retinal microcirculation is a complex system regulating blood flow.
  • Eicosanoids, derived from arachidonic acid, play crucial roles in vascular tone.
  • Understanding the vasoactive properties of specific eicosanoids is vital for retinal health.

Purpose of the Study:

  • To investigate the vasoactive effects of prostaglandin F2 alpha and PGH2 analogue U46619 on ex vivo bovine retinal microcirculation.
  • To determine the role of thromboxane A2 (TXA2) receptors in mediating these vascular responses.

Main Methods:

  • Bovine retinal microvascular endothelial cells were cultured to assess eicosanoid synthesis from 14C-arachidonic acid.
  • An ex vivo perfused bovine retinal microvascular model was used, cannulating the central retinal arteriole.

Related Experiment Videos

  • Vasoactive compounds were applied cumulatively, and microarteriole diameters were measured using a video camera system.

    • Main Results:

    • Bovine retinal microvascular endothelial cells synthesized PGE2, PGF2 alpha, PGI2, and a PGH2 analogue, with PGI2 being the most abundant.
    • Prostaglandin F2 alpha and U46619 induced dose-dependent vasoconstriction in retinal microvessels.
    • The vasoconstrictive responses to PGF2 alpha and U46619 were significantly attenuated by the specific TP (TXA2) receptor antagonist SQ 29,548.

    Conclusions:

    • Prostaglandin F2 alpha and U46619 are potent vasoconstrictors in the bovine retinal microcirculation.
    • These vasoactive effects are mediated, at least in part, through thromboxane A2 (TXA2) receptors.
    • The findings contribute to understanding the regulation of retinal blood flow by arachidonic acid metabolites.