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Colorimetric detection of immobilised PCR products generated on a solid support
A K Lockley1, C G Jones, J S Bruce
1Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK. andrew.lockley@nottingham.ac.uk
Nucleic Acids Research
|March 15, 1997
Abstract:
By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.