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B lymphocyte-specific, Cre-mediated mutagenesis in mice
R C Rickert1, J Roes, K Rajewsky
1Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany.
Nucleic Acids Research
|March 15, 1997
Summary
Researchers created new mice to study gene deletion in B lymphocytes. These CD19-Cre mice enable precise gene modification, showing high deletion efficiency in various B cell stages.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- The Cre/loxP system derived from P1 phage enables conditional gene deletion in mice.
- Targeted gene modification in specific cell types is crucial for understanding cellular functions.
Purpose of the Study:
- To develop a Cre/loxP system for selective gene modification in B lymphocytes.
- To generate and validate CD19-Cre transgenic mice for B cell-specific gene targeting.
Main Methods:
- Generation of CD19-Cre transgenic mice expressing Cre recombinase under the CD19 gene promoter.
- Crossed CD19-Cre mice with mice carrying a loxP-flanked gene (substrate).
- Assessed Cre-mediated deletion efficiency in different B lymphocyte populations.
Main Results:
- CD19-Cre mice demonstrated efficient Cre-mediated deletion of the loxP-flanked gene.
- Deletion efficiency was 75-80% in bone marrow-derived pre-B cells.
- Deletion efficiency increased to 90-95% in splenic B cells.
Conclusions:
- CD19-Cre mice are a valuable tool for conditional gene deletion in B lymphocytes.
- This system allows for precise genetic manipulation of B cells, facilitating research in immunology and related fields.