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Related Experiment Videos

Circularized Ac/Ds transposons: formation, structure and fate

V Gorbunova1, A A Levy

  • 1Plant Genetics Department, Weizmann Institute of Science, Rehovot, Israel.

Genetics
|April 1, 1997
PubMed
Summary

Circular Ac molecules form in maize, but their role as transposition intermediates is uncertain. Evidence suggests these circles, especially those with deleted ends, may be abortive excision products, potentially explaining Ac-loss.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Maize Ac/Ds elements transpose via a cut-and-paste mechanism.
  • Transposition intermediates remain largely uncharacterized.

Purpose of the Study:

  • Investigate the formation and role of circular Ac molecules during transposition.
  • Determine if circular Ac/Ds elements are transposition intermediates or abortive products.

Main Methods:

  • Analysis of circular Ac molecules in maize.
  • Construction of artificial circular Ds elements.
  • Introduction of circular Ds into tobacco protoplasts with Ac-transposase.

Main Results:

  • Circular Ac molecules with head-to-head joined ends were detected in maize.
  • Circular Ac molecules often contained deletions or insertions at the junction.
  • Artificial circular Ds elements with intact ends showed no efficient transposase-mediated integration in tobacco.

Conclusions:

  • Circular Ac molecules with deleted ends are likely abortive excision products.
  • Circular Ds elements with intact ends do not efficiently integrate, suggesting they are not standard transposition intermediates.
  • Ac circle formation may explain Ac-loss, impacting transposition models.

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