Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ras-independent transformation by v-Src

D T Aftab1, J Kwan, G S Martin

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of the influence of corneal properties and densitometry on applanation and rebound tonometry in primary open angle glaucoma.

Journal francais d'ophtalmologie·2023
Same author

Canadian Surgery Forum.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

Prevalence of intimate partner violence perpetration among military populations: A systematic review and meta-analysis.

Aggression and violent behavior·2020
Same author

The Tincture of the Chloride of Iron.

The American journal of dental science·2019
Same author

Antiseptics and Germicides in Dentistry.

The American journal of dental science·2019
Same author

Dissipation.

The American journal of dental science·2019

v-Src transformation can occur without Ras activation. This study shows v-Src transforms cells via Ras-independent pathways, challenging previous understandings of tyrosine kinase signaling.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncology

Background:

  • Receptor and nonreceptor tyrosine kinases signal through Ras, a GTPase.
  • v-Src, a tyrosine kinase, typically activates Ras, mediating cellular transformation.
  • Ras-dependent pathways are considered crucial for v-Src's biological effects.

Purpose of the Study:

  • To investigate whether Ras activation is essential for v-Src-mediated cellular transformation.
  • To determine if Ras-independent signaling pathways contribute to v-Src transformation.
  • To elucidate the role of Ras in v-Src's downstream signaling, including ERK2 activation.

Main Methods:

  • Expression of wild-type and mutant v-Src in Rat-2 and chicken embryo fibroblasts.
  • Utilizing dominant-negative Ras mutants to inhibit Ras function.

Related Experiment Videos

  • Assessing cellular transformation criteria and mitogen-activated protein kinase ERK2 activation.

    • Main Results:

    • Ras was not activated in Rat-2 fibroblasts transformed by wild-type v-Src or in chicken embryo fibroblasts transformed by a v-Src mutant (SRX5).
    • Dominant-negative Ras inhibited v-Src-induced ERK2 activation, a downstream target of Ras.
    • Despite inhibiting ERK2, dominant-negative Ras did not suppress v-Src-mediated cellular transformation.

    Conclusions:

    • v-Src can transform certain cell types independently of Ras activation.
    • Ras-independent signaling pathways are sufficient for v-Src-mediated transformation in specific cellular contexts.
    • This finding necessitates a re-evaluation of Ras's role in tyrosine kinase signaling and cellular transformation.