Related Experiment Video

Updated: Jun 30, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
08:33

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Developmental expression of the murine spliceosome-associated protein mSAP49

P Ruiz-Lozano1, P Doevendans, A Brown

  • 1Department of Medicine, University of California, San Diego, La Jolla, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|April 1, 1997
PubMed

Insights

We identified mSAP49, a protein crucial for spliceosome function, and observed its dynamic expression during mouse heart development. Its changing levels suggest a role in regulating cardiac gene splicing and development.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The spliceosome is a dynamic molecular machine essential for gene expression.
  • Spliceosome-associated proteins play critical roles in regulating splicing.
  • Understanding the roles of specific spliceosome components during development is vital.

Purpose of the Study:

  • To isolate and characterize the mouse homologue of human spliceosome-associated protein SAP49 (mSAP49).
  • To investigate the expression pattern of mSAP49 during mouse embryonic and cardiac development.
  • To explore the potential functional significance of mSAP49's dynamic cardiac expression.

Main Methods:

  • Isolation of mouse spliceosome-associated protein 49 (mSAP49).
  • In situ hybridization to analyze mSAP49 mRNA distribution in mouse embryos.
  • Northern blot analysis to quantify mSAP49 mRNA levels in cardiac tissue.

Main Results:

  • mSAP49 possesses two RNA recognition motifs (RRMs) and a basic C-terminus.
  • mSAP49 expression is widespread in adult mice but lower in the heart.
  • Cardiac mSAP49 expression is developmentally regulated, with a peak during late embryogenesis and a decline in adults.

Conclusions:

  • mSAP49 exhibits a dynamic expression pattern during cardiac development.
  • This dynamic expression suggests mSAP49's involvement in regulating cardiac-specific splicing.
  • mSAP49 may influence RNA stabilization or translation during heart development.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...