Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes
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1Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
Abstract
Objective:
To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes.
Materials And Methods:
Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure super oxide anion.
Results:
IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10-100 micrograms/ml) countered superoxide induction caused by 20 ng/ml of IL-1.
Conclusions:
HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.