Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Valproic acid-induced changes in gene expression during neurulation in a mouse model

B C Wlodarczyk1, J C Craig, G D Bennett

  • 1National Veterinary Research Institute, Department of Pharmacology and Toxicology, Pulawy, Poland.

Teratology
|December 1, 1996
PubMed
Summary

Valproic acid exposure alters gene expression during embryonic development, accelerating differentiation and potentially causing neural tube defects. This occurs through changes in cell proliferation, not increased cell death.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo ALX4 variant detected in child with non-syndromic craniosynostosis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2021
Same author

Corrigendum to "Digenic variants of planar cell polarity genes in human neural tube defect patients." Mol Genet Metab. 2018 May;124(1):94-100. doi:10.1016/j.ymgme.2018.03.005. Epub 2018 Mar 18. https://pubmed.ncbi.nlm.nih.gov/29573971/.

Molecular genetics and metabolism·2021
Same author

Effect on non-vascular outcomes of lowering LDL cholesterol in patients with chronic kidney disease: results from the Study of Heart and Renal Protection.

BMC nephrology·2017
Same author

Incidence and Predictors of Cancer Following Kidney Transplantation in Childhood.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Obesity and hypertension in Australian young people: results from the Australian Health Survey 2011-2012.

Internal medicine journal·2016
Same author

Use of electromyography to detect muscle exhaustion in finishing barrows fed ractopamine HCl.

Journal of animal science·2016

Area of Science:

  • Developmental Biology
  • Teratology
  • Molecular Genetics

Background:

  • Valproic acid (VPA) is a known teratogen linked to congenital defects.
  • Individual susceptibility to VPA's teratogenic effects varies due to genetic factors.
  • Neural tube defects (NTDs) are a significant concern in VPA-exposed pregnancies.

Purpose of the Study:

  • To investigate differential gene expression changes during valproate-induced NTDs in a mouse model.
  • To examine the roles of specific transcription factor and cell cycle checkpoint genes.
  • To elucidate the molecular mechanisms underlying VPA's teratogenic effects on neural tube closure.

Main Methods:

  • Utilized a mouse model to study valproate-induced NTDs.
  • Analyzed gene expression of transcription factors (Pax-3, Emx-1, Emx-2, c-fos, c-jun, creb) and cell cycle genes (bcl-2, p53, wee-1).

Related Experiment Videos

  • Compared gene expression profiles in VPA-exposed embryos versus controls at specific developmental stages.

    • Main Results:

    • VPA exposure accelerated developmental gene expression profiles in embryos.
    • Significant elevations in Emx-1, Emx-2, c-fos, c-jun, and creb mRNA were observed.
    • Overexpression of p53 and bcl-2 genes, favoring bcl-2, suggests altered cell proliferation is key.

    Conclusions:

    • Altered neuroepithelial cell proliferation rates, rather than apoptosis, underlie VPA-induced NTDs.
    • VPA exposure prematurely shifts the embryonic molecular profile from proliferation to differentiation.
    • Changes in multiple gene expressions collectively contribute to valproic acid-induced neural tube defects.