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Modulation of endoplasmic reticulum calcium pump expression during T lymphocyte activation
S Launay1, R Bobe, C Lacabaratz-Porret
1U348 INSERM, Institut Fédératif de Recherche Circulation Lariboisière, Hôpital Lariboisière, 8, rue Guy Patin, 75475 Paris Cedex 10, France.
The Journal of Biological Chemistry
|April 18, 1997
Summary
T cell activation significantly alters calcium pump expression. Combined drug treatment decreases one sarco-endoplasmic reticulum calcium ATPase (SERCA) isoform while increasing SERCA 2b, impacting calcium homeostasis.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Calcium mobilization is crucial for cell activation.
- Calcium transport ATPases regulate intracellular calcium levels.
- Sarco-endoplasmic reticulum calcium ATPase (SERCA) isoenzymes are key regulators.
Purpose of the Study:
- To investigate the modulation of SERCA isoenzyme expression during T lymphocyte activation.
- To understand the role of specific SERCA isoforms in activated T cells.
Main Methods:
- T lymphocyte cell lines were treated with phorbol myristate acetate and ionomycin.
- Expression levels of SERCA isoforms were analyzed using isoform-specific antibodies.
- Interleukin-2 expression and cyclosporine-A inhibition were assessed.
Main Results:
- Combined phorbol myristate acetate and ionomycin treatment decreased a specific SERCA isoform by ~90% and increased SERCA 2b by ~2-fold.
- Individual drug treatments had no significant effect.
- Down-modulation of the SERCA isoform correlated with interleukin-2 induction and was inhibited by cyclosporine-A.
Conclusions:
- T cell activation induces selective, cyclosporine-A-sensitive changes in SERCA isoform expression.
- These alterations signify a reorganization of calcium homeostasis in activated T cells.
- Findings may inform strategies for pharmacological modulation of lymphocyte function.