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Cloning and sequencing of a new 15-hydroxyprostaglandin dehydrogenase related mRNA

R Delage-Mourroux1, F Pichaud, J L Frendo

  • 1INSERM U 349, Hôpital Lariboisière, Paris, France.

Gene
|March 25, 1997
PubMed

Insights

Researchers identified a new mRNA variant, PGDH(rII), related to the prostaglandin-inactivating enzyme 15-PGDH. This finding in medullary thyroid carcinoma suggests alternative splicing regulates 15-PGDH gene expression.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is crucial for prostaglandin inactivation.
  • Previous work identified an mRNA isomer (PGDH(rI)) of 15-PGDH.
  • Medullary thyroid carcinoma (MTC) cell lines express mRNAs for both known 15-PGDH isomers.

Purpose of the Study:

  • To investigate the expression of novel 15-PGDH-related mRNAs in MTC.
  • To characterize a newly identified mRNA variant in TT cells and MTC.

Main Methods:

  • RNase protection assays were utilized to detect and confirm mRNA expression.
  • Analysis of mRNA sequences to identify variants and their characteristics.

Main Results:

  • A third 15-PGDH-related mRNA, termed PGDH(rII), was identified.
  • PGDH(rII) is 241 nucleotides shorter than the type-I 15-PGDH mRNA.
  • TT cells and MTC express the PGDH(rII) mRNA.

Conclusions:

  • The expression of PGDH(rII) in MTC suggests a more complex regulation of 15-PGDH.
  • Alternative splicing patterns are likely involved in the post-transcriptional regulation of the type-I 15-PGDH gene in MTC.

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