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Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5'-GGATG-3'
S Kh Degtyarev1, N A Netesova, M A Abdurashitov
1SibEnzyme, Novosibirsk, Russia.
Gene
|March 18, 1997
Abstract:
The gene for BstF5I-1 DNA-methyltransferase (MTase) from Bacillus stearothermophilus F5 (a FokI isoschizomer, recognizing 5'-GGATG-3') was cloned and its nucleotide (nt) sequence was determined. Analysis of deduced amino acid (aa) sequence shows that M x BstF5I-1 belongs to D21 class of MTases and has a little homology with M x FokI. M x BstF5I-1 modifies only the upper strand of recognition sequence (5'-GGATG-3').