Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Identification of pre-gamma-globin

R M Johnson¹, S Buck, H Schneider

¹Department of Biochemistry and Molecular Biology, Wayne State Medical School, Detroit, MI 48201, USA.

|March 1, 1997

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Novel mass spectral fragmentation of heptafluorobutyryl derivatives of acyl analogues of platelet-activating factor.

Journal of the American Society for Mass Spectrometry·2013

Same author

Characterization of Diacylglycerylphosphocholine Molecular Species by FAB-CAD-MS/MS: A General Method Not Sensitive to the Nature of the Fatty Acyl Groups.

Journal of the American Society for Mass Spectrometry·2013

Same author

N-Alkylnicotinium halides: A class of cationic matrix additives for enhancing the sensitivity in negative ion Fast-Atom bombardment mass spectrometry of polyanionic analytes.

Journal of the American Society for Mass Spectrometry·2013

Same author

Direct matrix-assisted laser desorption ionization mass spectrometric analysis of proteins immobilized on nylon-based membranes.

Journal of the American Society for Mass Spectrometry·2013

Same author

Quantitative assessment of cysteine and cystine in peptides and proteins following organomercurial derivatization and analysis by matrix-assisted laser desorption ionization mass spectrometry.

Journal of the American Society for Mass Spectrometry·2013

Same author

Cefetamet pivoxil treatment causes loss of carnitine reserves that can be prevented by exogenous carnitine administration.

The Journal of nutritional biochemistry·2004

Researchers identified a pre-gamma-globin in platyrrhine primates using high-performance liquid chromatography. This finding, linked to gene duplication and a specific cysteine residue, suggests a broader presence of glutathionyl gamma-globin across primate hemoglobins.

Area of Science:

Primate Hematology
Molecular Evolution
Protein Chemistry

Background:

Hemoglobin structure and function are critical for oxygen transport.
Gene duplication events can lead to variations in globin chains.
Post-translational modifications of hemoglobin can alter its properties.

Purpose of the Study:

To identify and characterize novel globin species in platyrrhine primate hemoglobin.
To investigate the role of gene duplication in hemoglobin evolution.
To determine the location and nature of modifications in pre-gamma-globin.

Main Methods:

High-performance liquid chromatography (HPLC) for protein separation.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for molecular identification.

Related Experiment Videos

Comparative analysis of globin gene structures in primates.

Main Results:

A distinct pre-gamma-globin species was identified in platyrrhine hemoglobins via HPLC.
The presence of pre-gamma-globin was facilitated by the functional inactivation of a duplicated gamma-globin gene.
MALDI-MS confirmed that a portion of pre-gamma-globin is glutathionyl gamma 2-globin, with glutathione attached at cysteine 93.

Conclusions:

The identification of pre-gamma-globin in platyrrhines provides insights into hemoglobin evolution.
The invariant nature of cysteine 93 across primates suggests that glutathionyl gamma-globin formation is a conserved phenomenon.
Further research is warranted to explore the functional implications of glutathionyl gamma-globin in various primate species.