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Related Experiment Videos

LDL- is a lipid hydroperoxide-enriched circulating lipoprotein

A Sevanian1, G Bittolo-Bon, G Cazzolato

  • 1Department of Molecular Pharmacology and Toxicology, University of Southern California, School of Pharmcy, Los Angeles 90033, USA.

Journal of Lipid Research
|March 1, 1997
PubMed
Summary

A new study identifies a distinct LDL particle subclass (LDL-) enriched in lipid peroxides. This subclass, differing in fatty acid and amino acid composition, may originate from vascular oxidative events or dietary factors.

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Area of Science:

  • Biochemistry
  • Cardiovascular Science
  • Analytical Chemistry

Background:

  • Low-density lipoprotein (LDL) modification is implicated in atherosclerosis.
  • Oxidative status of LDL particles can vary significantly.

Purpose of the Study:

  • To characterize a subclass of LDL particles with greater electronegativity and oxidative status, termed LDL-.
  • To investigate the lipid peroxide content and compositional differences of LDL- compared to normal LDL (nLDL).

Main Methods:

  • Utilized a novel, highly sensitive single photon counting technique to measure lipid hydroperoxides in whole LDL particles.
  • Analyzed fatty acid composition of total LDL lipids and amino acid composition of apoB-100.
  • Assessed conjugated dienes, cholesterol oxidation products, TNBS reactivity, and electrophoretic mobility.

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Main Results:

  • LDL- particles are significantly enriched in lipid hydroperoxides and peroxidation products compared to nLDL.
  • LDL- exhibits lower proportions of polyunsaturated fatty acids and specific oxidizable amino acids (histidine, lysine) in apoB-100.
  • Observed differences in LDL- composition are consistent with oxidative modification and correlate with reduced TNBS reactivity and increased electrophoretic mobility.

Conclusions:

  • LDL- represents a major carrier of lipid hydroperoxides within plasma LDL.
  • The formation of LDL- may result from oxidative events within the vasculature or from dietary intake of peroxides.