Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-specific local structural variations in solution structures of d(CGXX'CG)2 and d(CAXX'TG)2

S L Lam1, S C Au-Yeung

  • 1Department of Chemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

Journal of Molecular Biology
|March 7, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Secular trend of growth in pre-school children in Singapore.

Asia Pacific journal of clinical nutrition·2013
Same author

Pregnancy luteoma presenting as ovarian torsion with rupture and intra-abdominal bleeding.

Singapore medical journal·2008
Same author

Clinics in diagnostic imaging (110). Right-sided tubal ligation clips complicated by the formation of an infected retention cyst.

Singapore medical journal·2006
Same author

Clinics in diagnostic imaging (106). Viable left tubal twin ectopic pregnancy.

Singapore medical journal·2005
Same author

Imaging findings in acute neck infection due to pyriform sinus fistula.

Annals of the Academy of Medicine, Singapore·2004
Same author

Clinics in diagnostic imaging (98). Heterotopic pregnancy with an intrauterine blighted ovum and a live tubal ectopic pregnancy.

Singapore medical journal·2004

This study introduces a new function, sigma LS, to describe DNA local structures based on NMR data. This function correlates well with DNA local stability, improving predictions for DNA sequences.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Structural Biology

Background:

  • Understanding DNA structure-function relationships is crucial for molecular biology.
  • Sequence-specific local structures influence DNA duplex stability and function.
  • Existing models may not fully capture the nuances of local DNA geometry.

Purpose of the Study:

  • To determine the solution structures of specific self-complementary DNA duplexes.
  • To introduce a novel sequence-specific local structure function (sigma LS).
  • To establish a correlation between local DNA structure and stability.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy was used to obtain structural data.
  • Interproton distances, torsion angles, and hydrogen bond constraints were applied.

Related Experiment Videos

  • A new local structure function, sigma LS, was developed based on helix twist, base roll, slide, and propeller twist.

    • Main Results:

    • Solution structures of eight self-complementary DNA molecules were elucidated.
    • The novel sigma LS function showed a strong correlation (r = 0.96) with base-pair-step relative local stability (delta G0 10).
    • Base morphology and nearest-neighbor base-pair interactions were identified as key factors in local DNA geometry.

    Conclusions:

    • The study provides the first experimental evidence linking relative local stabilities with sequence-specific local DNA structures.
    • The sigma LS function and the trimer-tetramer model offer a basis for predicting DNA sequence-specific local structures.
    • Local DNA geometry is influenced by both inter-strand purine-purine clashes and base morphology.