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Related Experiment Videos

Antigen processing and presentation by human trophoblast-derived cell lines

S J Gobin1, L Wilson, V Keijsers

  • 1Department of Immunohaematology and Blood Bank, Leiden University Hospital, The Netherlands.

Journal of Immunology (Baltimore, Md. : 1950)
|April 15, 1997
PubMed
Summary

Trophoblast cells, like JEG-3 and JAR, can process and present antigens using nonclassical MHC class I molecules. This indicates functional antigen processing and presentation machinery in these crucial placental cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Reproductive Biology

Background:

  • Trophoblast cells express nonclassical MHC class I molecules (HLA-G, HLA-E) but lack classical ones (HLA-A, HLA-B).
  • Expression of nonclassical MHC genes correlates with peptide transporter associated with antigen processing (TAP) gene expression.

Purpose of the Study:

  • To investigate the functional capacity of trophoblast-derived cell lines (JEG-3, JAR) in peptide processing, transport, and MHC class I loading.
  • To determine if trophoblasts can present endogenous peptides via MHC class I molecules.

Main Methods:

  • Utilized trophoblast-derived choriocarcinoma cell lines (JEG-3, JAR).
  • Performed immunoprecipitation to demonstrate physical interaction between TAP complex and HLA-G/HLA-E.
  • Conducted functional studies using HLA-A*0201-expressing transfectants with cytotoxic T cell clones.

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Main Results:

  • Demonstrated a physical interaction between the TAP complex and HLA-G/HLA-E.
  • HLA-A*0201 transfectants were recognized by allospecific and viral peptide-specific cytotoxic T cell clones.
  • Confirmed that trophoblast-derived cell lines can present endogenously derived peptides in the context of HLA-A*0201.

Conclusions:

  • The peptide transporter associated with Ag processing (TAP) complex and other antigen processing/presentation molecules are functionally active in trophoblast-derived cell lines.
  • Trophoblasts possess the capability to provide antigenic peptides for presentation by their naturally expressed nonclassical MHC class I molecules.