Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serum amyloid P component forms a stable complex with human C5b6

S F Barbashov1, C Wang, A Nicholson-Weller

  • 1Harvard-Thorndike Laboratory of the Beth Israel-Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|April 15, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody CR1-2B11 recognizes a non-polymorphic epitope of human CR1 (CD35).

Clinical and experimental immunology·2007
Same author

C5L2 receptor is not involved in C3a / C3a-desArg-mediated enhancement of bone marrow hematopoietic cell migration to CXCL12.

Leukemia·2005
Same author

A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1).

Clinical and experimental immunology·2005
Same author

Into the third century of complement research.

Immunology today·2001
Same author

Complement receptor 1/CD35 is a receptor for mannan-binding lectin.

The Journal of experimental medicine·2000
Same author

C5a-stimulated human neutrophils use a subset of beta2 integrins to support the adhesion-dependent phase of superoxide production.

Journal of leukocyte biology·2000
Same journal

Getting on your last nerve: IFNs and resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Antigen-presenting cancer-associated fibroblasts in murine pancreatic tumors differentially regulate T-cell phenotype and function.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

MHC class II on melanoma cells regulates the anti-tumor T cell response.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

ENPP1-dependent USP2 ubiquitination governs SQSTM1-mediated autophagy-dependent ferroptosis in trophoblast cells and exacerbates placental dysfunction in gestational diabetes mellitus.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

ER stress amplifies inflammation via a dual mechanism involving IκBζ-XBP1s synergism and Regnase-1 degradation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

The V158F polymorphism in human FcγRIIIa/CD16a defines opposing receptor responses when interacting with soluble immune complexes.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

A novel 30,000 m.w. protein was identified and microsequenced, revealing it to be serum amyloid P (SAP). This SAP protein binds to the C5b6 complement complex, influencing its interaction with C7 and neutrophil chemotaxis.

Area of Science:

  • Immunology
  • Complement System
  • Protein Biochemistry

Background:

  • The terminal complement pathway is crucial for immune defense.
  • The C5b6 complex is an intermediate in complement activation.
  • Serum amyloid P (SAP) is a known pentraxin protein found in serum.

Purpose of the Study:

  • To identify and characterize a 30,000 m.w. protein tightly bound to the C5b6 complement complex.
  • To investigate the functional consequences of this protein's interaction with C5b6 and downstream complement components.

Main Methods:

  • Activation of C7-depleted human serum with zymosan to form C5b6.
  • Isolation of the C5b6 complex using chromatography (lysine-Sepharose, anion exchange).
  • Protein identification via electrophoresis, electroblotting, and N-terminal microsequencing.

Related Experiment Videos

Main Results:

  • A 30,000 m.w. protein was identified as serum amyloid P (SAP).
  • SAP binds to C5b6 independently of calcium, unlike its typical binding.
  • The C5b6-SAP complex binds C7, forming a C5b67-SAP complex with retained hemolytic activity and neutrophil chemotactic properties.

Conclusions:

  • SAP is a novel component that binds to the C5b6 complement complex.
  • SAP binding to C5b6 influences the assembly and function of terminal complement complexes.
  • The interaction of SAP with complement complexes may modulate their biological fate and inflammatory roles.