Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capillary electrophoresis system for hemoglobin A1c determinations evaluated

C J Doelman1, C W Siebelder, W A Nijhof

  • 1Department of Clinical Chemistry and Hematology, Queen Beatrix Hospital, Winterswijk, The Netherlands.

Clinical Chemistry
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Management of high-grade neuroendocrine neoplasms: impact of functional imaging.

Endocrine-related cancer·2025
Same author

Efficacy of inflammation-based stratification for add-on celecoxib or minocycline in major depressive disorder: Protocol of the INSTA-MD double-blind placebo-controlled randomised clinical trial.

Brain, behavior, & immunity - health·2024
Same author

Non-esophageal eosinophilic gastrointestinal diseases: a narrative review.

Acta gastro-enterologica Belgica·2023
Same author

A single-centre analysis of post-colonoscopy colorectal cancer.

Acta gastro-enterologica Belgica·2021
Same author

Current practice in approaching controversial diagnostic and therapeutic topics in gastroenteropancreatic neuroendocrine neoplasm management. Belgian multidisciplinary expert discussion based on a modified Delphi method.

Acta gastro-enterologica Belgica·2020
Same author

Development of Precision Small-Molecule Proneurotrophic Therapies for Neurodegenerative Diseases.

Vitamins and hormones·2017

Capillary electrophoresis offers a new method for measuring Hemoglobin A1c (Hb A1c), crucial for diabetes management. This technique accurately determines Hb A1c levels, unaffected by common interfering substances or hemoglobin variants.

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Hemoglobin A1c (Hb A1c) is the standard analyte for assessing long-term glycemic control in diabetes mellitus patients.
  • Accurate Hb A1c measurement is vital for effective diabetes management and preventing complications.
  • Existing methods may be subject to interference from various factors, impacting reliability.

Purpose of the Study:

  • To introduce and evaluate capillary electrophoresis as a novel analytical technique for Hb A1c determination.
  • To assess the specificity and accuracy of capillary electrophoresis for Hb A1c measurement.
  • To determine if common interfering substances and hemoglobin variants affect the capillary electrophoresis method.

Main Methods:

  • Capillary electrophoresis was employed as the primary analytical technique for Hb A1c measurement.

Related Experiment Videos

  • The method's performance was evaluated by assessing its independence from labile Hb A1c fractions.
  • Interference from carbamylated hemoglobin, acetylated hemoglobin, and common hemoglobin variants (Hb F, Hb S, Hb C) was investigated.

    • Main Results:

    • The capillary electrophoresis method provides accurate Hb A1c determination.
    • The measurement is not influenced by the labile Hb A1c fraction.
    • Carbamylated hemoglobin, acetylated hemoglobin, and hemoglobin variants (Hb F, Hb S, Hb C) do not interfere with the analysis.

    Conclusions:

    • Capillary electrophoresis is a reliable and valuable analytical tool for clinical laboratories.
    • This technique offers an accurate method for measuring Hb A1c, essential for diabetes monitoring.
    • The method's robustness against common interferences enhances its clinical utility.